PCR System- Ann Arbor

Solicitation / RFP / RFQ

This solicitation is a Request for Quotation (RFQ) for a Real-Time PCR System for research purposes at the Abdel-Latif laboratory within the Department of Veterans Affairs. The system is intended to enhance capabilities for gene expression analysis, genotyping, and pathogen detection.

• Equipment: Real-Time PCR System with specific technical specifications including:

  • 96-well or 384-well block configuration with fast protocol capability.
  • White LED excitation source with OptiFlex technology (6 decoupled channels for 96-well or 5 coupled channels for 384-well).
  • VeriFlex Block technology with 6 independent temperature zones.
  • Integrated touchscreen interface with cloud connectivity.
  • Compliance with 21 CFR Part 11 guidelines.
  • 40-cycle runs in under 30 minutes (96-well) or 35 minutes (384-well).
  • Factory calibration for specified dyes (FAM, SYBR Green, VIC, NED, ABY, JUN, Mustang Purple, TAMRA, Cy5, and ROX).

• Tasks & Deliverables:

  • Equipment delivery and installation.
  • Installation setup documentation.
  • RNase P verification plate.
  • IT checklist for connectivity.
  • Training and knowledge transfer (orientation, user materials, support resources).
  • System validation and setup (IQ, OQ, IPV services).
  • Reporting: Installation and qualification report, performance verification documentation, system specifications, warranty information.

• Warranty & Support: One-year limited warranty from installation (or 15 months from shipment), optional post-warranty service contracts, telephone technical support.

• Contract Type: Firm-Fixed-Price.

• Delivery: FOB Destination to LTC Charles S. Kettles VA Medical Center, Ann Arbor, MI.

• Offer Due Date: April 28, 2026, at 09:00 AM EDT.

• Submission Method: Completed RFQ documents to percy.johnson2@va.gov.

• Evaluation: Not explicitly detailed in the provided text, but standard FAR clauses apply.

• Acquisition Type: Unrestricted.

• NAICS Code: 334516 (Analytical Laboratory Instrument Manufacturing).

• Size Standard: 1000 Employees.

• The solicitation incorporates FAR clauses by reference, including 52.212-1, 52.212-4, and 52.212-5.

• Specific items for pricing include the QS5 384 QPCR System, Taqman RNase P Fast 384W Verif Plate, and various consumables and service contracts.

Other / Unclassified

This document details the "Salient Characteristics" of a real-time PCR instrument. It outlines the instrument's capabilities, features, and specifications, covering its applications, hardware components (instrument block, onboard computer, touchscreen interface), performance metrics, software functionalities (desktop and online), and ecosystem integration. It also includes design compliance information and vendor service/support details.

This document is relevant to bidders as it describes the technical specifications and performance characteristics of a specific piece of equipment. Potential bidders may need to understand these specifications if the opportunity involves the procurement, maintenance, or integration of such a real-time PCR system. Key aspects for bidders include:

• Applications: Gene expression analysis, genotyping, pathogen detection, etc.
• Instrument Features: Block options (96/384-well), run times (<30 min), installation ease (no FSE required), bench space optimization, temperature range (4–99.9ºC), ramp rates, LED source lifetime.
• Software Capabilities: Stand-alone operation, touchscreen interface, data sharing via Connect platform, online analysis apps, desktop software for setup and analysis, security features (21 CFR Part 11 compliance).
• Performance: Amplicon measurement accuracy, dynamic range, multiplexing capabilities (up to 21 wavelength combinations), sensitivity (distinguishing 5,000 from 10,000 copies).
• Connectivity & Data Management: Connect platform integration, USB, LAN connectivity, onboard storage (10 GB).
• Compliance & Support: cUL, UL, CE, C-TICK, WEEE compliance; ISO 13485/9001 manufacturing; one-year limited warranty; optional service contracts; technical support.

Statement of Work (SOW) / Performance Work Statement (PWS)

The objective of this project is to acquire a Real-Time PCR System to enhance research capabilities at the Abdel-Latif laboratory. The system will support precise gene expression analysis, genotyping, and pathogen detection, aiming to improve the efficiency and innovation of discovery research.

The vendor is required to provide a Real-Time PCR System with specific technical specifications, including:

• A 96-well or 384-well block configuration with fast protocol capability.

• White LED excitation source with OptiFlex technology (6 decoupled channels for 96-well or 5 coupled channels for 384-well).

• VeriFlex Block technology with 6 independent temperature zones.

• Integrated touchscreen interface with cloud connectivity.

• Compliance with 21 CFR Part 11 guidelines.

• Completion of 40-cycle runs in under 30 minutes (96-well) or 35 minutes (384-well).

• Factory calibration for multiple dyes (FAM, SYBR Green, VIC, NED, ABY, JUN, Mustang Purple, TAMRA, Cy5, ROX).

• Equipment Delivery and Installation: Includes system delivery, installation documentation, RNase P verification plate, and an IT checklist.

• Training and Knowledge Transfer: Provision of orientation sessions, user training materials, and system operation/maintenance documentation.

• System Validation and Setup: Includes Instrument Qualification (IQ), Operational Qualification (OQ), and Instrument Performance Verification (IPV) services, along with documentation of optical and thermal accuracy.

• Installation and qualification report.

• Performance verification documentation.

• System specifications and validation documentation.

• Warranty information and service contract details.

• One-year limited warranty from installation (or 15 months from shipment).

• Optional post-warranty service contracts with next-business-day on-site repairs.

• Telephone technical support and field applications assistance.