Reagents for Ames, IA

Solicitation / RFP / RFQ

This solicitation (1232SA26Q0172) is a Request for Quotation (RFQ) for reagents compatible with the Rhapsody high-throughput (HT) microwell system. The government is procuring reagents for simultaneous single-nuclei sequencing (whole transcriptome) and single-nuclei ATAC-seq, with the ability to multiplex nuclei samples from porcine samples.

• Reagents compatible with the Rhapsody HT microwell system.

• Capable of simultaneous single-nuclei sequencing (whole transcriptome).

• Capable of simultaneous single-nuclei ATAC-seq.

• Ability to multiplex nuclei samples (sample tags) from porcine samples.

• Attachment 2 provides detailed specifications.

• Anticipated award type: Firm-fixed-price contract.

• The government intends to make one award.

• Submission: Contractors must submit pricing for all items to be considered responsive.

• Evaluation Criteria:

  • Price: Will be evaluated for fairness and reasonableness (FAR 12.204). Quantity price discounts and prompt payment discounts should be included.
  • Technical Acceptability: Products must meet or exceed all salient characteristics specified in the solicitation and include sufficient descriptive literature or documentation. Failure to meet requirements or provide adequate documentation will result in technical unacceptability.
  • Past Performance: May be evaluated using references, USDA data, and CPARS. Ratings include Acceptable, Neutral, or Unacceptable.

• Evaluation Method: Lowest price technically acceptable criteria. The lowest priced offer will be evaluated for technical acceptability. If not acceptable, the process continues with the next lowest priced offer.

• Quote Acceptance Period: Quotes must remain firm for 90 calendar days from the specified receipt date.

• Questions Deadline: March 15, 2026, 12:00 PM Pacific Time. Submit via email to Brian.Fien@usda.gov.

• This acquisition is an unrestricted requirement and is not set-aside for small business concerns.

• Applicable NAICS Code: 325413.

• Small Business Size Standard: 1250.

• All responsible sources may submit a quotation.

• The solicitation incorporates FAR and AGAR clauses and provisions by reference. Full text is available electronically at www.acquisition.gov.

• Attachment 1 contains solicitation terms and conditions, instructions, and evaluation criteria.

• Delivery is required 60 days after award, with early deliveries accepted. Shipping address: 1920 Dayton Avenue, Bldg 24, Ames, IA 50010.

• Information regarding agency protests and the USDA Ombudsman Program is provided.

Form / Attachment / Pricing Sheet

This document outlines the specifications for reagents and kits required for high-throughput single-nuclei whole-transcriptome and ATAC-seq analysis using the Rhapsody (HT) microwell system. It details the contractor's responsibilities and the specific items and quantities needed.

• Project: Reagents for multiplexing porcine nuclei for single-nuclei whole-transcriptome and ATAC-seq analysis.
• Contractor Requirements:
  • Must provide reagents and protocols compatible with the Rhapsody Scanner and HT-Express System (up to 8 lanes).
  • Reagent kits must be compatible with BD Rhapsody microwell-based cartridge technology with a multitier barcoding system (microwell/cartridge-based, not fluidics/droplet).
  • Must provide reagent kits compatible with both whole transcriptome analysis (WTA) and ATAC-seq assays, capable of multiplexing nuclei samples.
  • Must provide reagent kits capable of processing a wide range of cells/nuclei (hundreds to approximately 500,000) on one cartridge.
• Items and Quantities:
  • BD Rhapsody Enhanced Cartridge Reagent Kit v3 – Qty 2
  • Rhapsody ATAC-Seq Tagmentation Kit – Qty 2
  • Rhapsody cDNA Kit – Qty 2
  • ATAC-seq Multiomics Amplification Kit – Qty 1
  • Whole Transcriptome Analysis Kit – Qty 1
• Delivery: All items must be received onsite or per an agreed schedule within 15 business days of the award date.
• Place of Delivery: USDA, 1920 Dayton Avenue, Bldg 24, Ames, IA 50010.

This document is crucial for bidders to understand the specific technical requirements and the exact list of reagents and kits needed for the project. It directly informs the product offerings and quantities required for a compliant proposal. The delivery location and timeline are also key logistical considerations.